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. Author manuscript; available in PMC: 2008 Aug 24.
Published in final edited form as: J Mol Biol. 2007 May 25;371(4):959–970. doi: 10.1016/j.jmb.2007.05.056

Figure 4. F-actin severing activity of PTX-2.

Figure 4

F-actin (48 μM) in F-buffer was treated with the indicated molar ratio amounts of PTX-2, latrunculin B (LatB), reidispongiolide A (RedA), or halichondramide (Hal) for 30 min and then centrifuged at 20,000 × g for 30 min. The pellet of each sample following analysis by SDS-PAGE is shown. The pellets for G-actin (48 μM) in G-buffer without the addition of toxin are shown as a control for sedimentation of non-filamentous actin within the tube.