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. Author manuscript; available in PMC: 2008 Aug 15.
Published in final edited form as: Anal Biochem. 2007 Apr 20;367(2):247–258. doi: 10.1016/j.ab.2007.04.021

Fig. 4.

Fig. 4

Reproducibility of FP-based assay for RecA-DNA assembly under optimal conditions. The FP was measured for each well of a 96-well plate in the absence (open circles) or presence (open circles) of 500 μM ADP. Each data point indicates the mean FP (in millipolarization units, mP) for each well from three different experiments on three different days; the error bars indicate the standard deviation of each mean value. The overall means for the positive signal control (500 μM ADP) and negative background control (no inhibitor) were 35.0 ± 5.2 mP (CV = 15%) and 133 ± 3 (CV = 2%), respectively. The signal-to-background ratio was 3.8 and the signal-to-noise ratio was 18. The overall Z’ factor was 0.87.