Table 1.

Inhibition of RecA activities by nucleotide analogs as measured in microplate assays.

	Filament Assembly
Nucleotide Entry	% Inhibition (at 100 μM)	Kdapp (μM)
1	89 ± 5	60 ± 30
2	82 ± 2	44 ± 6
3	75 ± 7	50 ± 30
4	65 ± 2	100 ± 40
5	55 ± 4	340 ± 190
6	32 ± 3	≥ 500
	ATPase Activity
Nucleotide Entry	% Inhibition (at 100 μM)	Kic (μM)
6	98 ± 2	0.8 ± 0.1
2	34 ± 4	63 ± 7
1	11 ± 1	140 ± 50
3	7 ± 2	160 ± 20
5	6 ± 3	≥ 500
4	2 ± 2	≥ 500

Apparent dissociation constants (K_d^app) were assessed by measuring the extent of filament assembly in the FP assay over an inhibitor concentration range of 0 to 1000 μM (see Materials & Methods and Fig. 3D). ATP-competitive inhibition constants (K_ic) were assessed by measuring the apparent S_0.5 for ATP in the ATPase assay over an inhibitor concentration range of 0 - 1000 μM (see Materials & Methods and Fig. 5B). The identity of each nucleotide analog represented in the table is indicated by a specific numeral in the “Nucleotide Entry” column: (1) TTP (CAS# 18423-43-3); (2) N⁶-(1-naphthyl)ADP (see reference [15]); (3) 5-Me-UTP (CAS# 1463-10-11); (4) O⁶-Me-GTP (CAS# 99404-63-4); (5) 2′-O-Me-ATP (CAS# 2140-79-6); and (6) 2′-(or 3′)-O-(N-methylanthraniloyl)ATP (MANT-ATP, CAS# 85287-56-5).