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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Arch Biochem Biophys. 2007 Apr 5;464(1):36–47. doi: 10.1016/j.abb.2007.03.016

Table 3.

ABTS oxidation activity of recombinant, wild type OxDC and OxDC mutants.

ABTS Oxidation Activity (U/mg) −Peroxidase ABTS Oxidation Activity (U/mg) + Peroxidase Specific Activity (U/mg) Decarboxylase Activity
WT OxDC 0.07 ± 0.01 0.12 ± 0.02 79 ± 2
R92K 0.04 ± 0.01 0.08 ± 0.02 1.3 ± 0.4
E162D 0.07 ± 0.01 0.12 ± 0.02 40 ± 3
E162Q 0.6 ± 0.1 1.16 ± 0.03 0.4 ± 0.1
R270K 0.02 ± 0.01 0.03 ± 0.01 2.4 ± 0.4
E333D 0.04 ± 0.01 0.08 ± 0.01 4 ± 1
E333Q 0.07 ± 0.01 0.12 ± 0.01 0.4 ± 0.1
R92K/R270K 0.04 ± 0.03 0.07 ± 0.01 0.4 ± 0.1
E162Q/E333Q 0.04 ± 0.03 0.05 ± 0.01 0.7 ± 0.1