Fig. 3.
Screening of the pY peptide library against an SH2 domain. (a) Scheme showing the steps involved in colorimetric screening of the pY peptide library against a biotinylated MBP-SH2 domain. Key: MBP, maltose binding protein; SA, streptavidin; AP, alkaline phosphatase. (b) A photograph of a portion of the screened library (~50x magnification). A positive bead (turquoise colored) is shown.