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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Methods. 2007 Jul;42(3):207–219. doi: 10.1016/j.ymeth.2007.02.010

Fig. 4.

Fig. 4

Peptide sequencing by PED/MS. (a) Scheme showing the reactions involved in partial Edman degradation. Reagents and conditions: (a) 10–30:1 PITC:Fmoc-OSU; (b) TFA; (c) Repeat steps a and b 7 times; (d) 20% piperidine in DMF; and (e) CNBr in 70% TFA. (b) The MALDI mass spectrum of peptide TAFIpYDNVLNBBRM and its degradation products (from a single 90 μm bead). Key: M*, homoserine lactone.