Table 1.
Peptide sequences selected from the pY library against Src, Grb2, and Csk SH2 domainsa
| Src | Grb2 | Csk | |||||
|---|---|---|---|---|---|---|---|
| RMpYAII | QRpYSKI | NFpYYTL | FRpYTSM | HHpYENA | TYpYVNM | LYpYRNV | FCpYASA |
| FGpYAMI | WQpYVQI | FMpYAQV | VRpYTVM | HHpYENA | VTpYVNM | MYpYRNV | QKpYARI |
| ERpYAMI | HLpYDFI | HIpYSFV | HMpYDMM | VLpYQNA | LQpYVNM | VYpYTNV | ATpYAKI |
| AIpYAQI | HMpYDKI | VRpYSIV | FLpYEAM | SYpYVNA | NRpYVNM | HQpYVNV | CRpYAHI |
| RYpYAQI | HHpYDQI | HYpYSMV | HHpYEDM | SYpYVND | VYpYYNM | DYpYVNV | AGpYASM |
| NMpYAQI | TYpYDQI | YGpYTLV | HLpYEKM | VRpYVNF | GYpYMNQ | ILpYYNV | PRpYACP |
| RApYAQI | VYpYDVI | TWpYTQV | HVpYENM | HFpYQNH | PYpYMNQ | LYpYINY | VPpYSFP |
| HHpYARI | HMpYNEI | DGpYTVV | MLpYEMM | AKpYENI | QRpYQNQ | HGpYKNY | AApYSVV |
| TGpYARI | HYpYNII | LSpYVYV | IVpYEMM | QRpYKNI | VRpYQNQ | YYpYRNY | ARpYSMV |
| QVpYATI | AHpYNKI | EWpYMLV | HYpYEMM | LRpYENL | QVpYVNR | WMpYVNY | FHpYSRV |
| QNpYATI | FWpYNVI | HRpYDFV | HLpYEVM | NRpYMNL | VYpYVNT | SVpYSRV | |
| RGpYAVI | EFpYNVI | GYpYQVV | TSpYEVM | NRpYMNL | HVpYANV | HRpYTHC | |
| VYpYAVI | HApYNVI | KYpYAFM | PFpYNEM | VSpYQNL | YMpYANV | PYpYTQP | |
| IVpYAVI | YYpYQTI | HDpYAIM | IFpYNEM | VSpYQNL | FQpYENV | SEpYTRV | |
| SVpYAYI | WMpYQVI | NHpYAIM | PYpYNMM | WHpYVNL | HFpYENV | ALpYTRV | |
| RFpYTAI | HMpYQKI | RGpYAIM | YMpYNMM | NMpYANM | IFpYENV | KGpYTIL | |
| HTpYTFI | RGpYQLI | VVpYAIM | VFpYNSM | NRpYANM | ATpYENV | RApYRSH | |
| YGpYTII | HLpYFHI | GGpYAIM | TLpYNYM | ARpYENM | HYpYENV | ARpYRCI | |
| HHpYTII | FApYFYI | AYpYALM | HVpYMNM | HTpYMNM | LRpYENV | EYpYRRI | |
| RKpYTKI | RLpYHAI | IApYAYM | VVpYYYM | QHpYMNM | LRpYENV | RCpYRSI | |
| RApYTKI | ALpYHQI | VFpYSMM | SNpYYMM | NDpYMNM | FYpYHNV | RMpYRFP | |
| TFpYTLI | HIpYHKI | HApYSPM | EMpYYQM | TGpYMNM | HSpYKNV | YPpYRTP | |
| HQpYTLI | GVpYYAI | HLpYTAM | MYpYAEA | TGpYMNM | VFpYNNV | QLpYRPP | |
| PVpYTQI | GGpYYFI | HMpYTAM | VTpYAFA | AIpYQNM | VYpYNNV | HMpYRKV | |
| HTpYTQI | YDpYYLI | HMpYTEM | KFpYRTF | HYpYQNM | LYpYQNV | ||
| HMpYTRI | RLpYYQI | WHpYTEM | FSpYMYF | HLpYQNM | FNpYQNV | ||
| SYpYTRI | YGpYYVI | GYpYTFM | IGpYVLH | IRpYQNM | FVpYQNV | ||
| DMpYTSI | VFpYYYI | MYpYTIM | MFpYSMR | EMpYQNM | FVpYQNV | ||
| HYpYTTI | HHpYEEL | DYpYTLM | MYpYAMR | EMpYQNM | GWpYQNV | ||
| YRpYTYI | ARpYKKL | HFpYTMM | FHpYGRR | YGpYRNM | GWpYQNV | ||
| HEpYSII | RHpYQKL | SYpYTMM | KYpYKER | QLpYRNM | ILpYQNV | ||
| KApYSVI | SYpYTTL | HLpYTQM | HHpYVNM | VFpYQNV | |||
| MFpYSFI | HYpYTYL | IYpYTSM | RApYVNM | IYpYRNV | |||
a
Boldfaced sequences were resynthesized and tested for binding by SPR. M, norleucine; C, α-aminobutyric acid. The pY library screened against c-Src and Grb2 SH2 domains did not contain α-aminobutyric acid.