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. Author manuscript; available in PMC: 2008 Aug 10.
Published in final edited form as: J Mol Biol. 2007 Jun 2;371(2):308–316. doi: 10.1016/j.jmb.2007.05.077

Figure 1.

Figure 1

Design of the BU fusion protein. Ub (top) was inserted into a surface loop of Bn (bottom) between residues 66 and 67 (indicated by asterisk). Locations of mutated residues are shown in black. BU variants were cloned, expressed, and purified as described previously 2.