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. Author manuscript; available in PMC: 2008 Aug 10.

Published in final edited form as: J Mol Biol. 2007 Jun 2;371(2):308–316. doi: 10.1016/j.jmb.2007.05.077

Simulated Bn domain unfolding curves for three representative coupling regimes: (A) zero coupling (ΔG_X = 0), (B) strong coupling (ΔG_X = 20 kcal·mol⁻¹), (C) intermediate coupling (ΔG_X = 4 kcal·mol⁻¹). WT BU is depicted by the heavy black line. Variants in which the Ub domain is stabilized are shown in gray. Gray curves to the immediate left and far left of WT are mutants stabilized by 1 kcal·mol⁻¹ and 2 kcal·mol⁻¹, respectively. Variants in which the Ub domain is destabilized are shown in black. Black curves to the immediate right and far right of WT are mutants destabilized by 1 kcal·mol⁻¹ and 2 kcal·mol⁻¹, respectively. Free Bn is indicated by the dashed line. Curves are generated from the model in Figure 2 using the following parameters: ΔG_B = 7.5 kcal·mol⁻¹, m_B = 4.8 kcal·mol⁻¹·M⁻¹, ΔG_U = 5.2 kcal·mol⁻¹ (WT), m_B = 1.8 kcal·mol⁻¹·M⁻¹. Panel D shows the dependence Bn domain stability on Ub domain stability, as gauged by C_m values. The zero, strong, and intermediate coupling cases are represented by gray, solid black, and dashed black lines, respectively. Bn domain C_m values are derived from fitting the data in panels A–C to the two-state linear extrapolation equation. ΔC_m and ΔΔG^H2O values on the x-axes are relative to WT Ub.

Simulated Bn domain unfolding curves for three representative coupling regimes: (A) zero coupling (ΔG_X = 0), (B) strong coupling (ΔG_X = 20 kcal·mol⁻¹), (C) intermediate coupling (ΔG_X = 4 kcal·mol⁻¹). WT BU is depicted by the heavy black line. Variants in which the Ub domain is stabilized are shown in gray. Gray curves to the immediate left and far left of WT are mutants stabilized by 1 kcal·mol⁻¹ and 2 kcal·mol⁻¹, respectively. Variants in which the Ub domain is destabilized are shown in black. Black curves to the immediate right and far right of WT are mutants destabilized by 1 kcal·mol⁻¹ and 2 kcal·mol⁻¹, respectively. Free Bn is indicated by the dashed line. Curves are generated from the model in Figure 2 using the following parameters: ΔG_B = 7.5 kcal·mol⁻¹, m_B = 4.8 kcal·mol⁻¹·M⁻¹, ΔG_U = 5.2 kcal·mol⁻¹ (WT), m_B = 1.8 kcal·mol⁻¹·M⁻¹. Panel D shows the dependence Bn domain stability on Ub domain stability, as gauged by C_m values. The zero, strong, and intermediate coupling cases are represented by gray, solid black, and dashed black lines, respectively. Bn domain C_m values are derived from fitting the data in panels A–C to the two-state linear extrapolation equation. ΔC_m and ΔΔG^H2O values on the x-axes are relative to WT Ub.