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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Biomaterials. 2007 Jun 14;28(27):3918–3927. doi: 10.1016/j.biomaterials.2007.05.019

Figure 2.

Figure 2

Comparison of enzymatic degradation profiles of insoluble rat aortal elastin, and that isolated from HA4-treated and untreated cell layers. In each case, elastin pellets were digested with 1 ml of elastase (20 U/ml) over 8 hours at 37 °C (n = 9/ case/ time point). In panel A, values shown represent mean ± SD of fractions of the respective pre-digestion elastin pellet amounts retained after the designated period of enzymatic breakdown. Note that the error bars are too small to be visible on this plot.

Silver staining of soluble elastin peptides within the digestate solutions in each case show greater intensity, signifying greater amounts of elastin degradation products generated in the case of elastin from aortae and HA-free cultures than upon elastase treatment of elastin pellets from HA4-exposed cell layers.