Fig. 1. Stimulation of IFN-γ-mediated production of NO by CD40 ligation in BV-2 microglial cells.

Cells were treated with 2 μg/ml cross-linking antibodies against CD40 in the presence or absence of IFN-γ (25 units/ml) under serum-free conditions. At different hours of incubation supernatants were used for the nitrite assay using Griess reagent as described under “Materials and Methods.” Data are expressed as the mean of two separate experiments.