Fig. 3. Dose-dependent stimulation of iNOS expression by human recombinant CD40L in IFN-γ-treated BV-2 microglial cells.
Cells were treated with different concentrations of human recombinant CD40L (CD154) in the presence or absence of IFN-γ (25 units/ml) under serum-free conditions. A, after 24 h, supernatants were used for the nitrite assay. Data are mean ± S.D. of three different experiments. B, after 6 h of incubation, total RNA was isolated from cells, and Northern blot analysis for iNOS mRNA was carried out.