Fig. 9. Ligation of CD40 stimulates the activation of NF-κB but not of C/EBPβ in IFN-γ-stimulated BV-2 microglial cells.

Cells plated at 50–60% confluence in six-well plates were cotransfected with 1μg of either pBIIX-Luc (A) or pC/EBPβ-Luc (B) and 50 ng of pRL-TK. After 24 h of transfection, cells were stimulated with 25 units/ml IFN-γ and different concentrations of anti-CD40 for 6 h under serum-free conditions. Firefly (ff-Luc) and Renilla luciferase (r-Luc) activities were obtained by analyzing the total cell extract. Data are mean ± S.D. of three different experiments.