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The effect of dilution is observed on active Ca2+ transport into Sf9 vesicles. The activity was measured as described in Materials and Methods, using 12 μg/ml (solid symbols) or 4 μg/ml (open symbols) final protein concentrations. The slope of the linear regression with time is the Ca2+ transport activity: 0.0287 and 0.116 nmol Ca2+ (mg of protein h)−1 for high and low protein concentration, respectively.
