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. 1998 May 12;95(10):5573–5578. doi: 10.1073/pnas.95.10.5573

Figure 1.

Figure 1

Dopamine-induced recruitment of D1 receptors to the plasma membrane visualized by confocal microscopy. The images are represented by using a pseudo color scale, where blue is low intensity and yellow-red is high intensity. (Bar = 2 μm.) (a) Nonpermeabilized cell. (b–l) Cells treated as indicated, and then permeabilized with Triton X-100 after fixation and before incubation with the antibody. (b) Untreated. (c) Fenoldopam (1 μM), 1 min. (d) Fenoldopam (1 μM), 15 min. (e) Preincubation for 1 min with dopamine antagonist (SCH23390; 1 μM); incubation for 1 min with SCH23390 + 50 nM fenoldopam. (f) Preincubation for 1 min with 50 nM fenoldopam; incubation for 1 min with SCH23390 50 nM + fenoldopam. (g) Forskolin (100 nM). (h) Nitecapone (5 μM), 20 min. (i) Glu-dopa (5 μM), 10 sec. (j) Glu-dopa (5 μM), 1 min. (k) Preincubation for 1 min with 5 μM SCH23390; incubation for 1 min with SCH23390 + 5 μM glu-dopa. (l) Preincubation for 10 min with 20 nM bafilomycin; incubation for 1 min with 5 μM glu-dopa.