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. Author manuscript; available in PMC: 2008 Nov 3.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Aug 28;362(4):1026–1030. doi: 10.1016/j.bbrc.2007.08.087

Figure 1. EGF induced STAT5b phosphorylation.

Figure 1

(A) Schematic of STAT5b structure illustrating the conserved domains of the STAT proteins: amino-terminus (N-term), DNA binding domain (DBD), Src homolog domain 2 (SH2), transactivation domain (TAD). The Y and S indicate where tyrosine and serine phosphorylation sites are located. (B) SKBr3 cells stably expressing His-wtSTAT5b were treated with 100ng/mL EGF for the times indicated. Whole cell lysates were analyzed by immunoblotting with antibodies directed against phospho-S731 STAT5b (top), phospho-Y699 STAT5b (middle) and total STAT5b (bottom).