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. Author manuscript; available in PMC: 2008 Nov 3.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Aug 28;362(4):1026–1030. doi: 10.1016/j.bbrc.2007.08.087

Figure 2. Increased S731 phosphorylation of Y740/743F.

Figure 2

After transfection with His-STAT5b constructs, SKBr3 cells were either unstimulated (−) or stimulated (+) with 100ng/mL EGF for 15 minutes. STAT5b constructs were isolated using nickel-NTA magnetic agarose beads and analyzed by immunoblotting with anti-phospho-S731 STAT5b (top), anti-phospho-Y699 STAT5b (middle), or anti-STAT5b antibodies (bottom). Averages ± S.E. for wtSTAT5b and Y740/743F were calculated from densitometric analysis of three experiments to quantitate fold increase of Y740/743F con over wtSTAT5b con. The values of S731 phosphorylation per STAT5b are: wtSTAT5b con (1.00 ± 0.00); Y740/743F con (3.73 ± 0.97). Student’s t test was utilized to determine statistical significance between wtSTAT5b con and Y740/743F con. *, p=0.0475.