Table I. Comparison of eIF levels determined in this study with those reported by Ghaemmaghami et al. 15 and Von der Haar et al. 16.

eIF or its subunit	Gene	Ghaemmaghami copies/cella	Von der Haar copies/cellb	This study copies/cellc
eIF1	SUI1/YNL244C	None	250 000	81 000
eIF1A	TIF11/YMR260C	35 100	50 000
eIF2α	SUI2/YJR007W	17 100	180 000
eIF2β	SUI3/YPL237W	None	180 000	63 000
eIF2γ	GCD11/YER025W	20 800 ± 2840
eIF2Bα	GCN3/YKR026C	8 970	30 000
eIF2Bβ	GCD7/YLR291C	6 650 ± 951
eIF2Bγ	GCD1/YOR260W	9 530 ± 1 760		5 000
eIF2Bδ	GCD2/YGR083C	10 300 ± 1 000
eIF2Bε	GCD6/YDR211W	33 800 ± 6 700
eIF3a	TIF32/YBR079c	52 700 ± 4350
eIF3b	PRT1/YOR361C	47 500 ± 5600
eIF3c	NIP1/YMR309C	78 900 ± 6150		34 000
eIF3g	TIF35/YDR429C	GFP only	100 000
eIF3i	TIF34/YMR146C	2 400 ± 282		32 000
eIF3j	HCR1/YLR192C	17 900
Pci8p	PCI8/YIL071c	50.4
eIF4A1	TIF1/YKR059W	106 000	800 000
eIF4A2	TIF2/YJL138C	#
eIF4B	TIF3/YPR163C	24 000	155 000
eIF4E	TIF45/YOL139C	14 200	340 000
eIF4G1	TIF4631/YGR162W	9 760	17 500	9 400
eIF4G2	TIF4632/YGL049C	3 390		5 400
eIF5	TIF5/YPR041W	48 300 ± 13 300		67 000
L25	RPL25/YOL127W	None		(200 000)
${\text{tRNA}}_{\text{i}}^{\text{Met}}$	IMT1-4			640 000

^aRef. 15. The results based on a single experiment, unless SD is provided. None, no expression was observed.

^#a band detected, but not quantifiable. GFP only, detected expression in GFP microscopy, not in TAP western experiments.

^bRef. 16.

^cRecalculated from the relative values in Fig. 1A and B, based on the ribosome abundance of 200 000 copies/cell 24, which is shown in parenthesis.