Figure 5.

Sequence alignment of alpha-11 giardin and ten other members of the alpha giardin family, which possess 25–29% sequence identity. The secondary structural elements for alpha-11 giardin are indicated above the sequence alignment and colored according to repeats I–IV. Note that the first amino acid is a serine residue in the alpha-11 giardin sequence since the first methionine is absent due to the presence of an N-terminal GST tag that was introduced during cloning. Residues denoted as white letters on red background are strictly conserved. Residues denoted in red on a cyan background are homologous while residues denoted in black on a cyan background are non-homologous in this group. Ala134, Ala141 and Trp267 in the alpha-11 giardin sequence are highlighted in yellow background to stress location in the alpha-11 giardin sequence where it differs from all other alpha giardins. The sequence alignment was performed using CLUSTALW⁴⁵,⁴⁶ and the figure was prepared with the program ESPript⁴⁷.