Table 1.
Primer sequence, accession number, product size and annealing temperature of investigated genes
| Gene | Primers | Acc# | Size | Annealing |
| β-Actin | F5'GACATCCGCAAGGACCTCTA3' R5' ACGGAGTACTTGCGCTCAG3' |
BC102948 | 100 | * |
| PGDH | F5'GGAAAGCTGGACATCTTGGT3' R5'GCAAATTGCGTTCAGTCTCA3' |
BC102458 | 150 | 60°C |
| PGES-1 | F 5'GAACGACCCAGATGTGGAA3' R5'ATACGGCCCAGGAAGAAGAC3' |
NM_174443 | 153 | 59°C |
| AKR1B5 | F 5' GACCTTGGGTACCGTCACAT3' R5'TCTTTCTCACTGGGAATCACG3' |
S54973 | 150 | 59°C |
| PGE2 9- Reductase | F 5'AAGAAATGCAGCCGTGAACT3' R 5' GCTCCTTCTTCTGGGCTTTT 3' |
BC102943 | 155 | 59°C |
| COX-2 | F5'CATGATGTTCTTTGTTGGCATT3' R 5' GCGAATTCCAACTTTCCATC3' |
AF031698 | 154 | 60°C |
• Actin was amplified under the same conditions as gene of interest