Table 1. Fold change (± SEM) in microRNAs from four brain structures resulting from sleep loss.
Data are expressed as the average ratio (sleep-deprived/cage control) of normalized mean pixel intensities. The negative reciprocal is reported for ratios less than one. Each value was obtained from four independent determinations. Bold typed miRNA species meet all three exclusion criteria.
| miRNA | Hippocampus | Prefrontal Cortex | Somatosensory Cortex |
Hypothalamus |
|---|---|---|---|---|
| ambi_miR_13124 | 2.05 (± 0.78) *** | −2.01 (± 0.05) ** | −1.77 (± 0.13) *** | −1.12 (± 0.34) |
| ambi_miR_13193 | 3.31 (± 0.99) *** | −1.01 (± 0.32) | −1.37 (± 0.25) * | 2.62 (± 0.68) |
| ambi_miR_13232 | 1.06 (± 0.16) | −1.55 (± 0.20) ** | −1.31 (± 0.17) * | −1.33 (± 1.04) |
| ambi_miR_917 | 1.26 (± 0.18) ** | 1.17(± 0.12) | 1.65 (± 0.51) ** | 1.18 (± 0.48) |
| hsa_let_7a | 2.16 (± 0.92) ** | −2.55 (± 0.48) ** | −2.04 (± 0.04) ** | 1.12 (± 0.32) |
| hsa_let_7b | 1.53 (± 0.27) *** | −2.50 (± 0.15) ** | −1.57 (± 0.15) ** | −2.38 (± 0.03) ** |
| hsa_let_7c | 2.00 (± 0.45) *** | −1.36 (± 0.44) * | −1.32 (± 0.09) | −1.15 (± 0.35) |
| hsa_let_7d | 2.19 (± 0.58) *** | −1.28 (± 0.39) * | −1.51 (± 0.17) ** | 1.09 (± 0.34) |
| hsa_let_7e | 2.89 (± 0.81) *** | 1.22 (± 0.64) | 1.04 (± 0.27) | 2.32 (± 1.17) ** |
| hsa_miR_100 | 2.33 (± 0.59) *** | −1.43 (± 0.14) | −1.39 (± 0.32) | 1.63 (± 0.51) |
| hsa_miR_103 | 1.54 (± 0.16) *** | −1.67 (± 0.11) * | −1.06 (± 0.21) | 1.67 (± 0.42) * |
| hsa_miR_107 | 1.96 (± 0.32) ** | −1.68 (± 0.12) * | −1.09 (± 0.24) | 1.53 (± 0.27) * |
| hsa_miR_125a | 2.39 (± 0.56) *** | −1.69 (± 0.12) ** | −1.58 (± 0.02) * | −1.57 (± 0.41) * |
| hsa_miR_125b | 1.60 (± 0.22) *** | 1.17(± 0.16) | 1.15 (± 0.07) | 1.27 (± 0.60) |
| hsa_miR_126 | 1.63 (± 0.30) *** | −1.69 (± 0.15) ** | −2.06 (± 0.10) ** | −1.50 (± 0.17) |
| hsa_miR_127 | 2.00 (± 0.36) *** | −2.71 (± 0.08) *** | −1.55 (± 0.11) ** | −1.54 (± 0.17) |
| hsa_miR_128a | 2.23 (± 0.65) *** | 2.04 (± 0.56) ** | 1.01 (± 0.15) | 1.63 (± 0.55) |
| hsa_miR_128b | 1.47 (± 0.39) *** | −1.32 (± 0.25) ** | −1.70 (± 0.11) ** | −2.29 (± 0.12) ** |
| hsa_miR_132 | 1.82 (± 0.37) *** | −1.91 (± 0.12) ** | −1.71 (± 0.05) ** | −1.64 (± 0.13) * |
| hsa_miR_138 | 2.44 (± 0.72) *** | −3.35 (± 0.12) *** | −2.35 (± 0.19) *** | −1.82 (± 0.34) * |
| hsa_miR_139 | 2.49 (± 0.63) *** | 1.19 (± 0.41) | 1.08 (± 0.07) | 2.31 (± 0.50) * |
| hsa_miR_143 | 2.64 (± 0.60) *** | −1.49 (± 0.25) ** | −1.52 (± 0.32) ** | 1.19 (± 0.33) |
| hsa_miR_145 | 2.38 (± 0.42) *** | −1.95 (± 0.05) ** | 1.03 (± 0.25) | 1.09 (± 0.84) |
| hsa_miR_150 | 2.43 (± 0.44) *** | −1.23 (± 0.26) | 1.20 (± 0.19) | 2.54 (± 1.48) |
| hsa_miR_16 | 4.07 (± 1.58) *** | −1.63 (± 0.35) * | −1.56 (± 0.17) * | 1.70 (± 0.44) |
| hsa_miR_181a | 2.08 (± 0.34) *** | 1.01 (± 0.17) | 1.17 (± 0.17) | 1.51 (± 0.60) * |
| hsa_miR_181b | 3.14 (± 0.96) *** | −1.41 (± 0.31) * | −1.54 (± 0.13) * | 1.20 (± 0.56) |
| hsa_miR_185 | 2.00 (± 0.58) *** | −2.70 (± 0.13) ** | −1.94 (± 0.13) ** | −1.96 (± 0.20) ** |
| hsa_miR_191 | 1.66 (± 0.21) *** | −1.06 (± 0.11) | 1.21 (± 0.19) | 1.97 (± 0.68) ** |
| hsa_miR_22 | 1.72 (± 0.38) *** | 1.07 (± 0.17) | −1.09 (± 0.22) | 2.82 (± 0.52) |
| hsa_miR_222 | 1.57 (± 0.27) *** | −1.79 (± 0.03) ** | −1.14 (± 0.09) | 1.37 (± 0.21) |
| hsa_miR_23a | 3.60 (± 0.92) *** | 1.32 (± 0.03) * | 1.08 (± 0.22) | 2.43 (± 0.39) |
| hsa_miR_23b | 2.37 (± 0.43) *** | 1.40 (± 0.53) | 1.04 (± 0.19) | 1.41 (± 0.44) |
| hsa_miR_24 | 1.86 (± 0.40) *** | −2.34 (± 0.10) *** | −2.34 (± 0.14) *** | −1.27 (± 0.23) |
| hsa_miR_26a | 2.57 (± 0.66) *** | −1.32 (± 0.44) * | −1.69 (± 0.14) ** | −1.38 (± 0.42) |
| hsa_miR_27b | 2.62 (± 0.65) *** | −2.82 (± 0.06) ** | −2.79 (± 0.13) *** | −1.47 (± 0.19) |
| hsa_miR_29a | 2.69 (± 0.72) *** | 1.57 (± 0.84) * | −1.45 (± 0.11) | 1.45 (± 0.82) * |
| hsa_miR_30a_5p | 1.70 (± 0.38) *** | −2.16 (± 0.07) *** | −2.20 (± 0.08) * | −1.13 (± 0.47) |
| hsa_miR_30c | 2.34 (± 0.85) *** | 1.08 (± 0.27) | −1.02 (± 0.29) | 2.46 (± 0.53) |
| hsa_miR_30d | 1.64 (± 0.18) *** | −1.75 (± 0.21) | −1.09 (± 0.10) | 1.86 (± 0.25) * |
| hsa_miR_30e_5p | 2.08 (± 0.47) *** | 1.19 (± 0.34) * | 1.09 (± 0.30) | 3.31 (± 1.09) |
| hsa_miR_342 | 1.55 (± 0.19) *** | −1.27 (± 0.14) | −1.06 (± 0.02) | 1.74 (± 0.74) |
| hsa_miR_422b | 1.61 (± 0.06) ** | −2.05 (± 0.09) ** | −1.01 (± 0.26) | 2.24 (± 0.35) |
| hsa_miR_487b | 1.78 (± 0.38) *** | −1.51 (± 0.30) | −1.72 (± 0.31) ** | 1.31 (± 0.32) |
| hsa_miR_99a | 2.62 (± 0.66) *** | 1.17 (± 0.20) | 1.21 (± 0.27) | 1.63 (± 0.64) |
| hsa_miR_99b | 2.91 (± 0.96) *** | −1.78 (± 0.10) *** | −1.23 (± 0.23) | 1.10 (± 0.60) |
| mmu_let_7a | 2.31 (± 0.98) *** | −2.19 (± 0.48) *** | −1.92 (± 0.04) * | −1.10 (± 0.32) |
| mmu_miR_127 | 1.89 (± 0.33) *** | −2.38 (± 0.10) ** | −1.08 (± 0.13) | 1.29 (± 0.24) |
| mmu_miR_221 | 2.78 (± 0.79) *** | 1.05 (± 0.45) | 1.22 (± 0.20) | 1.25 (± 1.87) |
| mmu_miR_487b | 1.71 (± 0.34) ** | −1.80 (± 0.20) *** | −1.41 (± 0.15) * | 6.97 (± 3.54) |
| mmu_miR_99a | 2.13 (± 0.70) ** | −1.95 (± 0.04) *** | −2.05 (± 0.21) ** | −1.96 (± 0.11) * |
| rno_miR_422b | 1.61 (± 0.37) ** | −2.40 (± 0.08) *** | −1.55 (± 0.25) * | 1.97 (± 0.95) |
MiRNA species in normal type are included for comparison with the caveat that they are excluded by signal-to-noise, difference and/or ratio criteria
*
= p < 0.05
**
= p < 0.01
***
= p < 0.001.