Fig. 4.
Expression of the HKII mRNA transcript in cultured mesangial cells. SV40 MES 13 mesangial cells were treated with either 0, 10 ppb, or 50 ppb arsenic for 12 or 24 hours. Following treatment, RNA was extracted and used as a template for production cDNA via reverse transcriptase. Gene specific primers were used to amplify the HKII transcript, and the PCR product electrophoretically resolved on a 1% agarose gel for semi-quantitative analysis.