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. 2007 Oct 26;3(10):e153. doi: 10.1371/journal.ppat.0030153

Figure 1. Expression of APOBEC mRNAs in Different Cells.

Figure 1

Poly A+ RNA was isolated from individual cell types and APOBEC reading frames (A3B, A3C, A3F, and A3G) were amplified with primers specific for a single ORF. The specificity of the primers was verified by sequencing the PCR products. RT-PCR reactions for A3B, A3C, A3G, and A3F ORFs were analyzed by agarose gel electrophoresis. PCR templates were cDNAs for the indicated cell types or plasmids specific for A3B, A3C, A3G, or A3F (positive controls). Water was the nontemplate control (NTC), β-actin was the internal control, and reactions lacking reverse transcriptase (RT) were also used as control during cDNA preparation (unpublished data).