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. 2007 Oct 26;3(10):e153. doi: 10.1371/journal.ppat.0030153

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© 2007 Bourara et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The A3C gene cloned into the pTT-IRES-GFP lentiviral vector with an HA tag was expressed in 293T cells, and the protein detected with a polyclonal HA antibody.

(B) This construct was used to transfect SupT1 cells. After 48 h, transfection efficiency of the construct was monitored by FACS analysis of GFP expression.

(C) Transfected SupT1 cells were infected with the VSV-G-pseudotyped 210WW and NL4–3 HIV-1 and collected 24 h later. Viral DNA was amplified using the sensitive mutation assay, and population sequencing performed to analyze G-to-A mutation. The total number of G-to-A mutations in the protease region (positions 2255–2485) was counted separately for the GA and GG contexts. No mutations were detected after infection of SupT1 cells transfected with an empty vector. ND, non-detectable mutations.