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. 2007 Oct 26;3(10):e156. doi: 10.1371/journal.ppat.0030156

Figure 5. Kinetics of HIV-1 Sensitivity to CsA.

Figure 5

(A) CsA affects HIV-1 with the CA mutation T54A/N57A much longer than HIV-1 with WT CA. Human T cell Jurkat cells were infected with replication-competent HIV-1 virus encoding the WT envelope and the luciferase gene, which contains either the WT CA sequence or the T54A/N57A mutation. CsA was added to the well at indicated time points. Virus was added at 8 °C by spinolculation, and infections were synchronized by washing off the innocula and adding media at 37 °C. The relative percentage of infectivity was determined by assigning the luciferase value of the sample without CsA as 100% for each virus. White circles indicate WT, black circles the mutant (T54A/N57A). Similar results were observed in three independent experiments.

(B) Reverse transcription (RT) of T54A/N57A progresses similarly to that of WT. Similar experiments were done as above (A), except that nevirapine (50 μM) was added instead of CsA.