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. 2007 Jul 13;73(17):5435–5446. doi: 10.1128/AEM.00756-07

TABLE 1.

Oligonucleotides used in this studya

Gene, ORF, or vector in indicated expt Sense primer
Antisense primer
Size (bp) Reference/source
Name Sequence (5′→3′) Name Sequence (5′→3′)
RT-PCR and Northern blot analysis
    alr0750 Aalr0750F AGTTGCATTGGATCGCTCCGATAT Aalr0750R CTCTGCCCAAATCATGCACTATATG 467 5
    hoxE AhoxEF GTGGTGTGTACAGGTACGGC AhoxER TTACCTAAGACTTTGCCATC 188 5
    hoxF AhoxFF ACATTGGCAGACAAGAACGC AhoxFR TTGCTGACGCATCTTCAGGC 270 5
    alr0760 Aalr0760F TACCCTTCCAGTTCTCCAATCTTC Aalr0760R ATCTATCAACTTCATCTAAGCCG 482 5
    alr0761 Aalr0761F ACAGACAATTTCATGTTACAAGC Aalr0761R TATCAACTTGGATTTGATATCTAC 424 This work
    hoxU AhoxUF ACGACCAACTCATTAGCGC AhoxUR AGCCACGCAAACAGAAC 290 5
    alr0763 Aalr0763F GACAAGCAACAACCAGA Aalr0763R CTGGTGCTTCATCTAGGAA 417 This work
    hoxY AhoxYF TGAAGTTAGCAACAGTATGG AhoxYR AAGACTGATTCAGCACTACC 307 This work
    alr0765 Aalr0765F GCCACGAACAAGATGCT Aalr0765R TTTCGGCTCGTTGGTGT 417 This work
    hoxH AhoxHF GACTATTGCCTTGGATGCT AhoxHR GACAGAATATCTGGGTCGTT 513 This work
    rnpB rnpBF GACCAAACTTGCTGGATAACG rnpBR TTGCGAGGGCAGTTATCTATC 331 This work
    lexA LexAF TCGTCAGATGATGCAGGCGATGAA LexAR AGTGATGCGATCGCCACTTCGATA 424 This work
Cloning—lexA AlexAF2 GGATCCGAACGCCTAACAGAAGCGCA AlexAR2 AAGCTTTCACATATAACCGCGCCACA 618 This work
AlexAF3 GAATTCATGGAACGCCTAACAGAAGCG AlexAR3 GGATCCTCACATATAACCGCGCCACAC 618 This work
Identification of TSPs
    alr0750 Aalr0750R3 CTCTGCCCAAATCATGCACTATA This work
     Aalr0750R4 GTATTACCTGCGGTTGTT This work
    hoxU AhoxUR AGCCACGCAAACAGAAC This work
EMSA
    alr0750 fragment Aalr0750F2 AAGTAAGCTAGAAGGCGCTTGC Aalr0750R2 ATATCGGAGCGATCCAATGCAACT 499 This work
    hoxU fragment Aalr0761F3 GCATCTGGTTTAAATGGTTAC AhoxUR2 GCGCTAATGAGTTGGTCGT 535 This work
    pQE-30 pQEF CCCGAAAAGTGCCACCTG pQER GTTCTGAGGTCATTACTGG 334 This work
a

The underlined base pairs correspond to restriction sites.