TABLE 1.
Gene, ORF, or vector in indicated expt | Sense primer
|
Antisense primer
|
Size (bp) | Reference/source | ||
---|---|---|---|---|---|---|
Name | Sequence (5′→3′) | Name | Sequence (5′→3′) | |||
RT-PCR and Northern blot analysis | ||||||
alr0750 | Aalr0750F | AGTTGCATTGGATCGCTCCGATAT | Aalr0750R | CTCTGCCCAAATCATGCACTATATG | 467 | 5 |
hoxE | AhoxEF | GTGGTGTGTACAGGTACGGC | AhoxER | TTACCTAAGACTTTGCCATC | 188 | 5 |
hoxF | AhoxFF | ACATTGGCAGACAAGAACGC | AhoxFR | TTGCTGACGCATCTTCAGGC | 270 | 5 |
alr0760 | Aalr0760F | TACCCTTCCAGTTCTCCAATCTTC | Aalr0760R | ATCTATCAACTTCATCTAAGCCG | 482 | 5 |
alr0761 | Aalr0761F | ACAGACAATTTCATGTTACAAGC | Aalr0761R | TATCAACTTGGATTTGATATCTAC | 424 | This work |
hoxU | AhoxUF | ACGACCAACTCATTAGCGC | AhoxUR | AGCCACGCAAACAGAAC | 290 | 5 |
alr0763 | Aalr0763F | GACAAGCAACAACCAGA | Aalr0763R | CTGGTGCTTCATCTAGGAA | 417 | This work |
hoxY | AhoxYF | TGAAGTTAGCAACAGTATGG | AhoxYR | AAGACTGATTCAGCACTACC | 307 | This work |
alr0765 | Aalr0765F | GCCACGAACAAGATGCT | Aalr0765R | TTTCGGCTCGTTGGTGT | 417 | This work |
hoxH | AhoxHF | GACTATTGCCTTGGATGCT | AhoxHR | GACAGAATATCTGGGTCGTT | 513 | This work |
rnpB | rnpBF | GACCAAACTTGCTGGATAACG | rnpBR | TTGCGAGGGCAGTTATCTATC | 331 | This work |
lexA | LexAF | TCGTCAGATGATGCAGGCGATGAA | LexAR | AGTGATGCGATCGCCACTTCGATA | 424 | This work |
Cloning—lexA | AlexAF2 | GGATCCGAACGCCTAACAGAAGCGCA | AlexAR2 | AAGCTTTCACATATAACCGCGCCACA | 618 | This work |
AlexAF3 | GAATTCATGGAACGCCTAACAGAAGCG | AlexAR3 | GGATCCTCACATATAACCGCGCCACAC | 618 | This work | |
Identification of TSPs | ||||||
alr0750 | Aalr0750R3 | CTCTGCCCAAATCATGCACTATA | This work | |||
Aalr0750R4 | GTATTACCTGCGGTTGTT | This work | ||||
hoxU | AhoxUR | AGCCACGCAAACAGAAC | This work | |||
EMSA | ||||||
alr0750 fragment | Aalr0750F2 | AAGTAAGCTAGAAGGCGCTTGC | Aalr0750R2 | ATATCGGAGCGATCCAATGCAACT | 499 | This work |
hoxU fragment | Aalr0761F3 | GCATCTGGTTTAAATGGTTAC | AhoxUR2 | GCGCTAATGAGTTGGTCGT | 535 | This work |
pQE-30 | pQEF | CCCGAAAAGTGCCACCTG | pQER | GTTCTGAGGTCATTACTGG | 334 | This work |
The underlined base pairs correspond to restriction sites.