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. 2007 Jul 6;73(17):5553–5565. doi: 10.1128/AEM.00635-07

FIG. 4.

FIG. 4.

PCR results obtained for the analysis of region XII encompassing ORFs VVA1613 to VVA1636 for a subset of isolates. (A) PCR results obtained for a subset of isolates obtained for the primer pairs VVA1212F/VVA1213R, VVA1618F/VVA1623R, VVA1625F/VVA1625R, and VVA1625bF/VVA1627R targeting the 5′ insertion site and ORFs within the island. PCR results were positive for the clinical isolates and negative for the environmental isolates. Note that the primer pairs VVA1618F/VVA1623R and VVA1625bF/VVA1627R were only used for strains that were previously shown to be positive for region XII. (B) Location of the primer pairs used in this study. Region XII is shown in white. Large black arrows represent the core flanking chromosomal ORFs. Small black arrows refer to the primer pairs used within region XII. (C) A subset of strains analyzed with the primer pairs VVA1612F/VVA1612R and VVA1637F/VVA1639R targeting the 5′ and the 3′ core flanking ORFs, respectively. All isolates were positive for these primers pairs. (D) A subset of strains analyzed with the primer pair VVA1612F/VVA1637R designed within the core 5′ and 3′ genes, demonstrating that the genes are adjacent to one another. The molecular ladder used was a 1-kb DNA ladder (New England Biolabs).