TABLE 2.
Primers designed for this study
| Primer target and designation | Sequence (5′-3′) | Ta (°C)a | Expected product (bp) |
|---|---|---|---|
| Housekeeping genes | |||
| VVgyrBF | CTATCGTCATGGTGTGC | 48 | 1,805 |
| VVgyrBR | TCTCACCCAAACCTTTG | ||
| VVmdhF | TGTTATTGGTGCCGCTG | 55 | 918 |
| VVmdhR | TACTTTACGAAGTCCACGC | ||
| VVrpoDF | CGTTTCGATCATGTGTACCG | 60 | 1,340 |
| VVrpoDR | ACGCCGAAGTAAATGACCAC | ||
| VVGroEL1bF | GCGTGGTATCGACAAAGCTG | 55.9 | 702 |
| VVGroEL1bR | TGACGAATTTGAGCAACACG | ||
| VVpyrC | AATGGCATTGAAGCAGGAAC | 60 | 858 |
| VVpyrC | GGACAGTGTTTGGCATGATG | ||
| VVGro2F | GACGCACGTCAAAAGATGCTG | 59 | 1,542 |
| VVGro2R | GTGATCGGTGATCATCGCTTC | ||
| Region XII | |||
| VVA1612F | ACC CTG ATC GTT GGC TAC TC | 57 | 1,284 |
| VVA1612R | AAG TGG TGC GTC TTT GAG CAG C | ||
| VVA1612F | ACC CTG ATC GTT GGC TAC TC | 61 | 1,305 |
| VVA1613R | GGA GCG GTG TGA TGG TGT TG | ||
| VVA1616F | ACA TAG TCA GAA GCG GAC G | 56.1 | 2,161 |
| VVA1618R | TTA GGC ACT GGA AAT GGG | ||
| VVA1618F | AAG GTA ATG CTC CAG TCG GG | 57 | 4,240 |
| VVA1623R | TGT GCT TCC TCT TTG TTG C | ||
| VVA1625F | CGG TCT GTG GTT TAT CG | 47 | 1,822 |
| VVA1625R | TCG TTT CCA GTC GTC AC | ||
| VVA1625bF | TCG TAG AAC TGT GGG TAA GG | 57.3 | 3,979 |
| VVA1627R | CAT AAA CTG GCG TGG TAC AG | ||
| VVA1630F | AAG TAA ATC CAG TGG CTA GG | 54.9 | 2,799 |
| VVA1632R | GGA AGG GCA AAT CCT TTC | ||
| VVA1634F | TGA CAC CCA ACC TAG ACC AC | 55 | 1,364 |
| VVA1634R | ATT GAT GCC AAC CTG AG | ||
| VVA1636F | TGT CCA CGA CTT GAA CAC G | 56 | 1,547 |
| VVA1637R | AAC ATC AAC CAG CGA GTC GAA C | ||
| VVA1637F | GCT TAT TGA CCA ACG TG | 52 | 1,631 |
| VVA1639R | CGG TTG AAA CTG ACG AG | ||
| VVA1612bF | TGT GGA GAG CGG CAA GAT CAA G | 61 | 1,200 |
| VVA1637R | AAC ATC AAC CAG CGA GTC GAA C |
a
Ta, annealing temperature.