TABLE 1.
Peroxidase and laccase activities (towards veratryl alcohol and ABTS, respectively), benzyl alcohol oxidation, and final pH in liquid surface cultures of 26 Coprinus spp. after 21 days of culturea
| Coprinus species | Strain | ABTS oxidation in agar platesb | Peroxidase activity (U liter−1)c | Laccase activity (U liter−1)d | Benzyl alcohol oxidatione | pH of the medium after 3 wk |
|---|---|---|---|---|---|---|
| Coprinus alcalinus | C 149-1 | + | ND | Y | 8.37 | |
| Coprinus aureogranulatus | CBS 973.95 | + | ND | N | 7.70 | |
| Coprinus atramentarius | K 119 | + | ND | N | 6.29 | |
| Coprinus clastophylus | DSMZ 8306 | + | ND | Y | 7.41 | |
| Coprinus comatus | CCB 053 | + | ND | N | 6.64 | |
| Coprinus disseminatus | CBS 506.78 | + | ND | N | 7.61 | |
| Coprinus domesticus | CBS 378.90 | + | ND | Y | 8.04 | |
| Coprinus erythrocephalus | C 022-1 | + | ND | Y | 6.9 | |
| CBS 534.87 | + | ND | Y | 7.86 | ||
| Coprinus laanii | CBS 476.70 | − | ND | N | 7.35 | |
| Coprinus marculentus | CBS 433.86 | − | ND | Y | 7.89 | |
| Coprinus micaceus | DSMZ 1704 | + | ND | N | 7.84 | |
| Coprinus miser | CBS 680.70 | + | ND | N | 7.27 | |
| Coprinus narcoticus | CBS 681.70 | + | ND | Y | 8.02 | |
| Coprinus phaeosporus | CBS 894.70 | + | ND | N | 8.14 | |
| Coprinus picaceus | CBS 790.84 | + | ND | Y | 8.00 | |
| Coprinus psydromorbird | CBS 209.89 | + | ND | N | 6.21 | |
| Coprinus radians | DSMZ 888 | + | 178 ± 24 | 71 ± 12 | Y | 8.30 |
| Coprinus silvaticus | CBS 588.84 | + | ND | Y | 7.74 | |
| Coprinus sp. | Zi 12 | − | ND | N | 7.70 | |
| Z 3 | + | ND | Y | 7.86 | ||
| Coprinus sterquilinus | DSMZ 3341 | + | ND | Y | 6.55 | |
| Coprinus truncorum | CBS 640.66 | + | ND | N | 8.16 | |
| Coprinus tuberosus | CBS 590.80 | + | ND | N | 8.24 | |
| Coprinus verticillatus | DSMZ 3397 | + | 14 ± 8 | 388 ± 39 | Y | 8.04 |
| Coprinus xanthothrix | DSMZ 4916 | + | ND | Y | 7.95 |
The medium contained 30 g liter−1 soybean flour, and the initial pH was 6.7. Values given represent means ± standard deviations for three replicates.
+, dark violet coloring around and below the fungal mycelium; −, no coloring reaction (growth period, 7 to 14 days).
Peroxidase activity was determined by the oxidation of veratryl alcohol to veratraldehyde at pH 7 (41).
Laccase activity was measured by the oxidation of ABTS at pH 4.5 after 21 days (only in the case of C. radians and C. verticillatus). ND, not determined.
Benzyl alcohol (1.8 mM) was added just after inoculation and was quantified by HPLC after 3 weeks. Y, benzyl alcohol conversion of >10% and detection of benzaldehyde and/or benzoic acid; N, benzyl alcohol conversion of <10%, with detection of neither benzaldehyde nor benzoic acid.