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. 2007 Jun 29;73(17):5477–5485. doi: 10.1128/AEM.00026-07

TABLE 3.

Kinetic parameters of purified CrP II from Coprinus radiansa

Substrate Km (μM) kcat (s−1) kcat/Km (s−1 M−1)
Veratryl alcohol 88 34 3.86 × 105
Benzyl alcohol 635 176 2.77 × 105
ABTS 49 123 2.51 × 106
DMP 342 2 5.85 × 103
Naphthalene 584 15 2.57 × 104
H2O2 1,201 471 3.92 × 105
a

Reactions were performed with various substrate concentrations at pH 4.5 (ABTS and DMP) or pH 7 (aryl alcohols and naphthalene). Constants for H2O2 were determined in the presence of 5 mM benzyl alcohol.