TABLE 3.
Substrate | Km (μM) | kcat (s−1) | kcat/Km (s−1 M−1) |
---|---|---|---|
Veratryl alcohol | 88 | 34 | 3.86 × 105 |
Benzyl alcohol | 635 | 176 | 2.77 × 105 |
ABTS | 49 | 123 | 2.51 × 106 |
DMP | 342 | 2 | 5.85 × 103 |
Naphthalene | 584 | 15 | 2.57 × 104 |
H2O2 | 1,201 | 471 | 3.92 × 105 |
Reactions were performed with various substrate concentrations at pH 4.5 (ABTS and DMP) or pH 7 (aryl alcohols and naphthalene). Constants for H2O2 were determined in the presence of 5 mM benzyl alcohol.