FIG. 2.
Sites of Tn917 insertions. (A) Autoradiogram from a Southern blot analysis of the restriction digests of total DNA from E. faecalis strains following hybridization with 32P-labeled pTV1-OK. Lane 1, lambda DNA digested with HindIII and EcoRI; lane 2, HindIII-digested pTV1-OK; lane 3, HindIII-digested E. faecalis AR01/DGVS. HindIII-digested E. faecalis mutants: lane 4, DGRM2; lane 5, DGRM5; lane 6, DGRM12. (B) Downward-pointing arrows indicate the sites of Tn917 insertions for E. faecalis strains as follows: 2, DGRM2; 5, DGRM5; 12, DGRM12; 7349, JH2-7349. Tn917 insertions for strains DGRM2, DGRM5, and DGRM12 were located within the traB gene of pJM01, while a Tn917 insertion for strain JH2-7349 was located within the traB gene of pPD1. The number of amino acids (A.A.) for each ORF is in parentheses.