. 2007 Jul 13;73(17):5507–5515. doi: 10.1128/AEM.01013-07

TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristic(s)	Source or reference
E. coli MC1061	F⁻araD139 (ara-leu)7696 galE15 galK16 (lac)X74 rspL (Str^r) hsdR2 (r_k⁻ m_k⁺) mcrA mcrB1	7
E. faecalis
AR01/DGVS	AR01/DG cured of pJM02 (Vm^r Em^r) Tc^r Bc^r	36
DGRM2	AR01/DGVS traB::Tn917 Em^r Tc^r Bc^r Ml^r	36
DGRM5	AR01/DGVS traB::Tn917 Em^r Tc^r Bc^r Ml^r	36
DGRM12	AR01/DGVS traB::Tn917 Em^r Tc^r Bc^r Ml^r	36
AR01/DGVS/pAM401	AR01/DGVS harboring pAM401 Tc^r Bc^r Cm^r	This study
DGRM2/pAMCL6	DGRM2 harboring pAMCL6 Em^r Tc^r Bc^r Sp^r Ml^s	This study
DGRM5/pAMCL6	DGRM5 harboring pAMCL6 Em^r Tc^r Bc^r Sp^r Ml^s	This study
DGRM12/pAMCL6	DGRM12 harboring pAMCL6 Em^r Tc^r Bc^r Sp^r Ml^s	This study
JH2-2	Glycopeptide-susceptible strain commonly used for gene transfer experiments with Enterococcus	22
JH2-7349	JH2-2 harboring pPIT7349 Em^r	This study
JH2-7013	JH2-2 harboring pPIT7013 Em^r	This study
Plasmids
pTV1-OK	repA(Ts)-pWV01Ts aphA3 Tn917 Km^r Em^r	19
pCL1921	Low-copy-number vector; St^r Sp^r, 4.6 kb	33
pCL6	pCL1921 harboring 6.8-kb insert from AR01/DGVS containing traB; St^r Sp^r	This study
pAM401	E. coli-E. faecalis shuttle vector; Cm^r Tc^r, 10.4 kb	61
pAMCL6	pAM401 harboring pCL1921 and 6.8-kb insert from AR01/DGVS containing traB; Tc^r	This study
pPIT7349	pPD1::Tn917 derivative with insertion in traB	16
pPIT7013	pPD1::Tn917 derivative with insertion in orfY	16