Skip to main content
. 1998 May 12;95(10):5584–5589. doi: 10.1073/pnas.95.10.5584

Figure 1.

Figure 1

Subcellular localization and function of RGS4 in yeast cells requires the amino-terminal 33 residues of the protein. (A) A schematic diagram of the primary structure of RGS4 protein. Shaded box represents the RGS homology domain. Numbers below the box correspond to amino acid residues in the protein. (B) Effects of deletion mutations in RGS4-GFP on its ability to inhibit pheromone signaling and its subcellular localization. For each pair, the left panel shows halo assays measuring pheromone response of an sst2Δ mutant (BC180) expressing the indicated constructs fromYCp50, a single-copy vector. The right panel represents confocal images of wild-type yeast cells (SWY518) harboring the same constructs in pVT102U (multicopy) vector. Numbers in parentheses in front of GFP represent the corresponding amino acids of RGS4. (C) Confocal images of cells expressing GFP fusions containing the first 12 or first 33 aa of RGS4.