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C-terminal prenylation/palmitoylation sequences functionally replace the N-terminal 33 aa of RGS4. Halo assays of an sst2Δ mutant (BC180) expressing the indicated constructs from the pVT102U vector are shown in the left column. Confocal images of SWY518 cells harboring the corresponding constructs are shown in the right column. The smaller halo size for functional RGS4 in this figure compared with Fig. 1 is because of higher protein expression from a multicopy plasmid.
