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. 1998 May 12;95(10):5584–5589. doi: 10.1073/pnas.95.10.5584

Figure 5.

Figure 5

Analysis of the N-terminal domain of RGS4. (A) Mutation of cysteine residues within the amino terminus of RGS4 has no effect on the ability of RGS4 to inhibit pheromone response or its localization. The column on the left represents halo assays of an sst2Δ mutant (BC180) expressing the indicated constructs in a low-copy (YCp50-based) vector. The column on the right shows confocal images of wild-type cells harboring the respective constructs in a high-copy (pVT102U-based) vector. RGS4-GFP represents the entire coding region of RGS4 fused to GFP, whereas (1–33)-GFP represents the first 33 aa of RGS4 fused to GFP. (B) Sequence of the first 33 aa of RGS4 protein. Basic amino acids are indicated in bold letters. (C) Confocal images of SWY518 cells expressing the indicated constructs in the high-copy vector. Numbers in parentheses represent the first 22 (Left) or first 33 (Center) aa of RGS4 fused to GFP. In Δ(13–29)RGS4-GFP, the indicated residues are deleted from an otherwise full-length protein.