TABLE 2.
Results of mouse bioassays at 45 to 48 days postinoculation to assess viability of T. gondii oocysts after exposure to CUV
| Oocyst dose | No. of mice | CUV exposure dose (mJ/cm2) | IFATc | Immunohistochemistry
|
Parasite isolation | |
|---|---|---|---|---|---|---|
| Reader 1 | Reader 2 | |||||
| Preliminary tests | ||||||
| Expt 1 | ||||||
| 105 | 3 | 0 | 2a | 3 | 3 | 2b |
| 105 | 3 | 100 | 0 | 0 | 0 | 0 |
| 105 | 3 | 200 | 0 | 0 | 0 | 0 |
| 105 | 3 | 500 | 0 | 0 | 0 | 0b |
| 105 | 3 | 1,000 | 0 | 0 | 0 | 0b |
| Expt 2 | ||||||
| 105 | 3 | 0 | 3 | 3 | 3 | 3 |
| 105 | 3 | 20 | 2 | 2 | 2 | 2 |
| 105 | 3 | 40 | 0 | 0 | 0 | 0 |
| 105 | 3 | 80 | 0 | 0 | 0 | 0 |
| 105 | 3 | 100 | 0 | 0 | 0 | 0 |
| Confirmation tests | ||||||
| Expt 3 | ||||||
| 104 | 3 | 0 | 3 | 3 | 3 | 3 |
| 104 | 3 | 160 | 0 | 0 | 0 | 0 |
| 104 | 3 | 186 | 0 | 0 | 0 | 0 |
| 104 | 3 | 228 | 0 | 0 | 0 | 0 |
| 104 | 3 | 335 | 0 | 0 | 0 | 0 |
| 104 | 3 | 412 | 0 | 0 | 0 | 0 |
| 104 | 3 | 731 | 1 | 0 | 0 | 1 |
| Expt 4 | ||||||
| 104 | 3 | 0 | 3 | 3 | 3 | 3 |
| 104 | 3 | 160 | 1 | 0 | 1 | 1 |
| 104 | 3 | 186 | 0 | 0 | 0 | 0 |
| 104 | 3 | 228 | 0 | 0 | 0 | 0 |
| 104 | 3 | 335 | 1 | 0 | 1 | 1 |
| 104 | 3 | 412 | 0 | 0 | 0 | 0 |
| 104 | 3 | 731 | 0 | 0 | 0 | 0 |
a
One mouse died at PID 12; antibody immune response was not detectable.
b
One culture became contaminated with bacteria and was discarded.
c
IFAT with a positive cutoff value of 1:40.