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. 2007 Sep 27;104(41):16062–16067. doi: 10.1073/pnas.0704534104

Fig. 4.

Fig. 4.

ChIP analysis of the occupancy of promoter and ORF of ADH1, MET17, and VTC3 in DST1 and MED31 mutant contexts. (A) ChIP analysis of Pol II in DST1, dst1-(1–265), dst1-R200A, and MED31 strains grown in rich glucose medium at 37°C. Immunoprecipitation signal over input signal is represented in arbitrary units. (B) ChIP analysis of Pol II in DST1, dst1-(1–265), and dst1-R200A in med31-Δ background grown in rich glucose medium at 37°C. (C) ChIP analysis of TBP in DST1, dst1-(1–265), dst1-R200A, and med31-Δ strains grown in rich glucose medium at 37°C. (D) ChIP analysis of TFIIS binding to promoter or ORF of ADH1, MET17, and VTC3 in med31-Δ DST1 and med31-Δ dst1-R200A strains. Cells were cultured to log phase in glucose-rich medium at 30°C and then shifted at 37°C for 30 min before cross-linking. Anti-TFIIS polyclonal antibodies were used for immunoprecipitation.