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. 2007 Oct 2;104(41):16164–16169. doi: 10.1073/pnas.0702896104

Fig. 2.

Fig. 2.

BART Cluster 1 miRNAs target the LMP1 3′ UTR. (A) Schematic showing the location of predicted LMP1 target (tL) sites for BART 13-5p, 16, 17-5p, and 1-5p miRNAs. The LMP1 gene (open bar) is numbered according to GenBank accession no. X01995. Light shading, ORF. (B) Wild-type (wt) and mutant (mut) LMP1 3′ UTR target sequences of BART3-5p, 16, 17-5p, and 1-5p miRNAs that were cloned into luciferase reporters. (C) Luciferase activity of LMP1 target constructs in the presence of BART miRNAs. HeLa cells were transfected with luciferase reporters together with the indicated miRNA. The luciferase activity was normalized to β-gal activity. Fold change in luciferase activity of the wild-type LMP1 construct (black bars) was calculated relative to that of the mutated construct (white bars). Data shown are the mean ± SD from three separate experiments.