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St-domain is active in primary cardiac cells. Plasmids ANGCAT, STANGCAT (carrying deletion of St-domain), and MSTANGCAT (carrying a substitution mutation of the St-domain) were constructed and used in a transient transfection experiment. The CAT reporter activity was evaluated before (0 hr) and after (1 and 2 hr) of treatment with AngII peptide (1 × 10−7 M) of rat primary cardiac cells. Each bar represents the mean of four experiments.
