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STAT–St-domain interaction on GMSA with extracts from SHR. (A) Cardiac muscle tissues obtained at 13, 23, and 33 weeks of age from both WKY and SHR were used to prepare nuclear extract for analysis by GMSA with St-domain as probe. W, normotensive strain WKY; S, hypertensive strain SHR. (B) Nuclear extracts from heart of WKY rat were prepared after AngII (10−7 M) treatment of WKY rat and compared for binding to St-domain with untreated WKY rat (−) on GMSA as in A.
