Fig. 2.

Hfq is required for S. typhimurium LSMMG-induced phenotypes in RWV culture. (A) The survival ratio of WT and isogenic hfq, hfq 3′Cm, and invA mutant strains in acid stress after RWV culture in the LSMMG and 1 × g positions is plotted (P < 0.05, ANOVA). (B) Fold intracellular replication of S. typhimurium strains hfq 3′Cm and Δhfq in J774 macrophages after RWV culture as above. Intracellular bacteria were quantitated at 2 h and 24 h after infection, and the fold increase in bacterial numbers between those two time periods was calculated (P < 0.05, ANOVA).