Skip to main content
. 2007 Oct 3;104(41):16317–16322. doi: 10.1073/pnas.0703344104

Fig. 3.

Fig. 3.

Overexpression of spatially restricted βCaMKII-F90G in dentate gyrus layer in younger Tg animals (age 60–85 days PN). (A) In situ hybridization using PA probe EJ shows no discernible signal from brain slices from WT littermates of βCaMKII-F90G Tg (at the age of PN 60–80 days). (B) In situ hybridization using PA probe EJ shows dominant expression of βCaMKII-F90G mRNA in the Tg dentate gyrus layer over CA1 at PN 80 days. (C) Normal input–output curve of Tg mice in perforant pathway in the hippocampal slices (WT, n = 6 slices per three mice; Tg, n = 11 slices per five mice). (D) Normalized paired-pulse responses in dentate gyrus of the hippocampal slices from Tg mice (WT, n = 14 slices per seven mice; Tg, n = 14 slices per five mice). (E) βCaMKII-F90G overexpression does not alter the plasticity responses in the hippocampal Schaffer collateral pathway in younger animals aged 60–85 days PN. LTP induced by a tetanic stimulation (one train, 100 Hz, 1 s per train) in Tg slices (154 ± 8.8% at minutes 41–45; n = 6 slices per four mice) was no different from that of WT slices (152 ± 5.6% at minutes 41–45; n = 7 slices per five mice; P = 0.856 compared with Tg mice). (F) βCaMKII overexpression alters LTP in the hippocampal perforant pathway. LTP induced by a tetanic stimulation (six trains, 100 Hz, 1 s per train, interval 60 s, duration 0.1 ms) in Tg slices (131 ± 1.35% at minutes 81–90; n = 8 slices per four mice) was significantly smaller than that of WT slices (171 ± 12.3% at minutes 81–90; n = 13 slices per seven mice; P = 0.006 compared with Tg mice). (G) NM-PP1 (0.5 μM in artificial cerebrospinal fluid) restored LTP of the βCaMKII-F90G Tg mice in the hippocampal medial perforant pathway. LTP induced by a tetanic stimulation (same as in F) in Tg slices with NM-PP1 (179 ± 15.5% at minutes 81–90; n = 10 slices per seven mice) were restored to the level of WT slices with NM-PP1 (177 ± 25.2% at minutes 81–90; n = 6 slices per five mice; P = 0.49 compared with Tg mice with NM-PP1). All values are mean percentage compared with baseline response ± SEM. Statistical differences were evaluated with a t test. All mice used were 75–85 days old.