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. 1987 Dec;53(12):2922–2928. doi: 10.1128/aem.53.12.2922-2928.1987

Comparison of four membrane filter methods for fecal coliform enumeration in tropical waters.

J Santiago-Mercado 1, T C Hazen 1
PMCID: PMC204223  PMID: 3324970

Abstract

Four membrane filter methods for the enumeration of fecal coliforms were compared for accuracy, specificity, and recovery. Water samples were taken several times from 13 marine, 1 estuarine, and 4 freshwater sites around Puerto Rico, from pristine waters and waters receiving treated and untreated sewage and effluent from a tuna cannery and a rum distillery. Differences of 1 to 3 orders of magnitude in the levels of fecal coliforms were observed in some samples by different recovery techniques. Marine water samples gave poorer results, in terms of specificity, selectivity, and comparability, than freshwater samples for all four fecal coliform methods used. The method using Difco m-FC agar with a resuscitation step gave the best overall results; however, even this method gave higher false-positive error, higher undetected-target error, lower selectivity, and higher recovery of nontarget organisms than the method using MacConkey membrane broth, the worst method for temperate waters. All methods tested were unacceptable for the enumeration of fecal coliforms in tropical fresh and marine waters. Thus, considering the high densities of fecal coliforms observed at most sites in Puerto Rico by all these methods, it would seem that these density estimates are, in many cases, grossly overestimating the degree of recent fecal contamination. Since Escherichia coli appears to be a normal inhabitant of tropical waters, fecal contamination may be indicated when none is present. Using fecal coliforms as an indicator is grossly inadequate for the detection of recent human fecal contamination and associated pathogens in both marine and fresh tropical waters.

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Selected References

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  1. Baker R. M., Singleton F. L., Hood M. A. Effects of nutrient deprivation on Vibrio cholerae. Appl Environ Microbiol. 1983 Oct;46(4):930–940. doi: 10.1128/aem.46.4.930-940.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carrillo M., Estrada E., Hazen T. C. Survival and enumeration of the fecal indicators Bifidobacterium adolescentis and Escherichia coli in a tropical rain forest watershed. Appl Environ Microbiol. 1985 Aug;50(2):468–476. doi: 10.1128/aem.50.2.468-476.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dufour A. P., Strickland E. R., Cabelli V. J. Membrane filter method for enumerating Escherichia coli. Appl Environ Microbiol. 1981 May;41(5):1152–1158. doi: 10.1128/aem.41.5.1152-1158.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evison L. M., James A. A comparison of the distribution of intestinal bacteria in British and East African water sources. J Appl Bacteriol. 1973 Mar;36(1):109–118. doi: 10.1111/j.1365-2672.1973.tb04078.x. [DOI] [PubMed] [Google Scholar]
  5. Fuentes F. A., Biamon E. J., Hazen T. C. Bacterial chemotaxis to effluent from a rum distillery in tropical near-shore coastal waters. Appl Environ Microbiol. 1983 Dec;46(6):1438–1441. doi: 10.1128/aem.46.6.1438-1441.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fujioka R. S., Hashimoto H. H., Siwak E. B., Young R. H. Effect of sunlight on survival of indicator bacteria in seawater. Appl Environ Microbiol. 1981 Mar;41(3):690–696. doi: 10.1128/aem.41.3.690-696.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grabow W. O., du Preez M. Comparison of m-Endo LES, MacConkey, and Teepol media for membrane filtration counting of total coliform bacteria in water. Appl Environ Microbiol. 1979 Sep;38(3):351–358. doi: 10.1128/aem.38.3.351-358.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hagler A. N., Mendonça-Hagler L. C. Yeasts from marine and estuarine waters with different levels of pollution in the state of rio de janeiro, Brazil. Appl Environ Microbiol. 1981 Jan;41(1):173–178. doi: 10.1128/aem.41.1.173-178.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hazen T. C., Esch G. W. Effect of effluent from a nitrogen fertilizer factory and a pulp mill on the distribution and abundance of Aeromonas hydrophila in Albemarle Sound, North Carolina. Appl Environ Microbiol. 1983 Jan;45(1):31–42. doi: 10.1128/aem.45.1.31-42.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hood M. A., Ness G. E. Survival of Vibrio cholerae and Escherichia coli in estuarine waters and sediments. Appl Environ Microbiol. 1982 Mar;43(3):578–584. doi: 10.1128/aem.43.3.578-584.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jiwa S. F., Krovacek K., Wadström T. Enterotoxigenic bacteria in food and water from an Ethiopian community. Appl Environ Microbiol. 1981 Apr;41(4):1010–1019. doi: 10.1128/aem.41.4.1010-1019.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lavoie M. C. Identification of strains isolated as total and fecal coliforms and comparison of both groups as indicators of fecal pollution in tropical climates. Can J Microbiol. 1983 Jun;29(6):689–693. doi: 10.1139/m83-112. [DOI] [PubMed] [Google Scholar]
  13. McCambridge J., McMeekin T. A. Effect of solar radiation and predacious microorganisms on survival of fecal and other bacteria. Appl Environ Microbiol. 1981 May;41(5):1083–1087. doi: 10.1128/aem.41.5.1083-1087.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McFeters G. A., Stuart D. G. Survival of coliform bacteria in natural waters: field and laboratory studies with membrane-filter chambers. Appl Microbiol. 1972 Nov;24(5):805–811. doi: 10.1128/am.24.5.805-811.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pagel J. E., Qureshi A. A., Young D. M., Vlassoff L. T. Comparison of four membrane filter methods for fecal coliform enumeration. Appl Environ Microbiol. 1982 Apr;43(4):787–793. doi: 10.1128/aem.43.4.787-793.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sjogren R. E., Gibson M. J. Bacterial survival in a dilute environment. Appl Environ Microbiol. 1981 Jun;41(6):1331–1336. doi: 10.1128/aem.41.6.1331-1336.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Valdes-Collazo L., Schultz A. J., Hazen T. C. Survival of Candida albicans in tropical marine and fresh waters. Appl Environ Microbiol. 1987 Aug;53(8):1762–1767. doi: 10.1128/aem.53.8.1762-1767.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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