Figure 1.

Kinetic of penetration of mAb F4.1 (50 μg/ml) into 3T3 cells cultured at 37°C or 4°C. At indicated time, cells were trypsinized, counted, washed, and lyzed, and IgG2a mAb in lysates was quantified by ELISA by reference to a standard curve established with F4.1 mAb.