Figure 2.
Transfer of various macromolecules by mAbs or their fragments or by a derived peptide. (A) HEp-2 cells cultured overnight with J20.8 F(ab′)2 fragments coupled to PO (50 μg/ml). (B) PtK2 cells cultured with biotinylated P3 (20 μg/ml) for 1 h and after fixation incubated with streptavidin-PO (5 μg/ml). (C) Biotinylated P3 (1.4 μg) was incubated with streptavidin-PO (10 μg) in 35 μl of PBS for 20 min at room temperature, diluted to 250 μl with complete medium, and added to PtK2 cell culture for 2 h. After fixation, PO was detected with ME-DAB. Arrow shows a dividing cell.