Table 2.
Reactivities assessed by ELISA of penetrating and nonpenetrating mAbs with a panel of antigens
| mAbs | dsDNA | ssDNA | Histone | DNase | Actin | Myosin | Tubulin | Ars | Ph-Ox | TNP | Kd (×10−9) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| C2.4 (+) | 0.070* | 3.5 | 5 | 10 | 5 | 5.6 | 3 | 1.3 | 1.2 | 5 | 90 |
| C12.24 (+) | 0.022 | 10 | 5 | 10 | 5 | 5 | Neg. | 1.2 | 3 | Neg. | 80 |
| F4.1 (+) | 0.040 | 2 | 5 | 15 | 1.8 | 5 | 6 | 1.2 | 3 | 20 | 90 |
| J20.8 (+) | 0.066 | 60 | 16 | 30 | 16 | 2.5 | 3.5 | 1.8 | 5 | 30 | 350 |
| G14 (+) | 0.066 | 1.6 | 6 | 5 | 6 | 1.5 | 2 | 0.4 | 1 | 4 | 770 |
| F14.6 (+) | 0.060 | 0.8 | 0.6 | 10 | 0.6 | 0.2 | 1.8 | 0.5 | 0.45 | 3 | 150 |
| A2.1 (−) | 0.066 | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | 1,000 |
| H9.3 (−) | 0.140 | Neg. | 30 | Neg. | 30 | Neg. | Neg. | Neg. | Neg. | Neg. | 1,500 |
+, Penetrating mAb; −, nonpenetrating mAb; Neg., negative; ssDNA, single-stranded DNA; Ars: arsonate; Ph-Ox, phenyloxazolone; TNP, trinitrophenyl. All haptens were coupled to ovalbumin. The Kd values were calculated by using an inhibition assay (see Ref. 12).
*
Reactivity is expressed as the mAb concentration in μg/ml giving an OD414 of 0.200 in 2 h.