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DALP. DALP employs two separate PCRs, each containing one of the primers labeled, for each sample. After separation in denaturing polyacrylamide gel, bands shared by the two reactions are products with different primers at both ends. These bands are compared with those of other samples in order to find the polymorphic bands among the samples. Polymorphic bands can be excised from the gel and sequenced directly using either of the primers.
