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A, B Isolation and characterization of a Dunning AT-2 prostate-cancer cell line transduced to express lacZ as a model TAA. A Following retrovirus tranduction, lacZ expression was measured by FACS-gal assay; cells expressing high levels of β-galactosidase were selected as shown using the cell sorter and were used for subsequent immunization studies. B Class I MHC expression was confirmed in AT-2 cells using mAb OX-18
