Figure 6.

PS and cyclodextrins. (a) Effect of γ-cyclodextrin on a low (1 μM) concentration of PS during the steady-state response of an oocyte to 30 μM GABA. Co-application with 0.5 mM γ-cyclodextrin (γ-CD) completely reversed PS inhibition. (b) The effect of γ-cyclodextrin at a saturating concentration of 100 μM PS. Arrows in (a) and (b) indicate the time points from which measurements were taken. (c) The concentration–response curve of PS was shifted to the right in the presence of γ-cyclodextrin compared with the curve in the absence of 0.5 mM γ-cyclodextrin. The solid line indicates a fit of the Hill equation to the average data points shown in the Figure. IC₅₀ of PS in the presence of γ-cyclodextrin shifted from 0.8 μM (baseline) to 27 μM (in the presence of 0.5 mM γ-cyclodextrin) (n=15).