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Comparison of pSia-Luc constructs in different regions of the embryo. Luciferase activity was measured in embryos microinjected into ventral animal (va), dorsal animal (da), ventral vegetal (vvg), and dorsovegetal (dvg) blastomeres with −833pSia-Luc (A and B), −296pSia-Luc (B and D), −245pSia-Luc (C), and −179pSia-Luc and −135 pSia-Luc (D). Experimental data are expressed as the means from triplicate samples ± SD. One representative experiment is shown in each graph.
